scriptcap m 7 g capping system (Epicentre Biotechnologies)

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Epicentre Biotechnologies scriptcap m 7 g capping system
Scriptcap M 7 G Capping System, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scriptcap+m+7+g+capping+system/scriptcap+m7g+capping+system/pmc11530239-307-10-20
Average 90 stars, based on 1 article reviews

scriptcap m 7 g capping system - by Bioz Stars, 2026-10

90/100 stars

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In Vitro:

Article Title: The activation of the decapping enzyme DCP2 by DCP1 occurs on the EDC4 scaffold and involves a conserved loop in DCP1
Article Snippet: Reactions were stopped by adding up to 50 mM EDTA and analyzed on PEI cellulose thin-layer chromatography plates (Merck) in 0.75 M LiCl (1 μl/sample). .. The in vitro synthesized RNA (127 nucleotides) probe was labeled with [γ- 32 P]GTP using the ScriptCap m 7 G Capping System and the ScriptCap 2’- O -Methyltransferase kit (EPICENTRE Biotechnologies). ..

Synthesized:

Labeling:

other:

Article Title: Differential Roles of Hsp70 and Hsp90 in the Assembly of the Replicase Complex of a Positive-Strand RNA Plant Virus
Article Snippet: If required, capped transcripts were prepared using the ScriptCap m 7 G capping system according to the manufacturer's instruction (Epicentre Biotechnologies, Madison, WI).

Spectrophotometry:

Article Title: Direct and specific chemical control of eukaryotic translation with a synthetic RNA–protein interaction
Article Snippet: Assembly PCR (30 cycles) was performed with Phusion DNA polymerase (New England Biolabs) according to the manufacturer's instructions, annealing at 65°C for 20 s and extending at 72°C for 25 s. Unpurified PCR products were transcribed with the MEGAScript T7 kit (Ambion), precipitated with an equal volume of 7.5 M LiCl/50 mM EDTA, washed with 70% ethanol and redissolved in water. .. The mRNA products were capped with the ScriptCap m 7 G capping system (Epicentre Biotechnologies) or the Vaccinia Capping System (New England Biolabs), precipitated with an equal volume of 7.5 M LiCl/50 mM EDTA, washed with 70% ethanol and redissolved in RBB [50 mM Tris–HCl, pH 8.0, 50 mM KCl, 5 mM MgCl 2 , 5% (v/v) glycerol, 0.05% (v/v) Tween-20]. mRNA concentrations were determined by measuring absorbance at 260 nm on a Nanodrop ND-1000 spectrophotometer (ThermoFisher Scientific). .. For cell-free translation with rabbit reticulocyte lysate (RRL), the mRNA concentration was adjusted to 133 nM in RBBD (RBB plus 1 mM dithiothreitol and 10 μg/ml bovine serum albumin, New England Biolabs) and refolded by heating at 65°C for 2 min and incubating at room temperature for 10 min. TetR(B) with N-terminal T7 and C-terminal His 6 tags, and revTetR-S2 with a C-terminal His 6 tag were purified from Escherichia coli as previously described ( ).

Review